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RESEARCH PRODUCT

FERULIC ACID INHIBITS OXIDATE STRESS AND CELL DEATH INDUCED BY Ab OLIGOMERS: IMPROVED DELIVERY BY SOLID LIPID NANOPARTICLES.

Pasquale PiconeMaria L. BondiPasquale PiconeMaria L. BondiGiovanna MontanaAndreina BrunoGiovanna PitarresiGaetano GiammonaMarta Di Carlo

subject

Coumaric AcidsMitochondrionmedicine.disease_causeBiochemistryAntioxidantsFerulic acidchemistry.chemical_compoundAlzheimer DiseaseCell Line TumorSolid lipid nanoparticlemedicineHumansViability assayMembrane Potential MitochondrialNeuronsAmyloid beta-PeptidesCell DeathbiologyCytochrome cCytochromes cFERULIC ACID Ab OLIGOMERS LIPID NANOPARTICLES DELIVERY SYSTEM.General MedicineLipidsPeptide FragmentsRecombinant ProteinsMitochondriaOxidative StressBiochemistrychemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDrug deliveryBiophysicsbiology.proteinNanoparticlesPeroxiredoxinOxidative stress

description

Oxidative stress and dysfunctional mitochondria are among the earliest events in AD, triggering neurodegeneration. The use of natural antioxidants could be a neuroprotective strategy for blocking cell death. Here, the antioxidant action of ferulic acid (FA) on different paths leading to degeneration of recombinant beta-amyloid peptide (rAbeta42) treated cells was investigated. Further, to improve its delivery, a novel drug delivery system (DDS) was used. Solid lipid nanoparticles (SLNs), empty or containing ferulic acid (FA-SNL), were developed as DDS. The resulting particles had small colloidal size and highly negative surface charge in water. Using neuroblastoma cells and rAbeta42 oligomers, it was demonstrated that free and SLNs-loaded FA recover cell viability. FA treatment, in particular if loaded into SLNs, decreased ROS generation, restored mitochondrial membrane potential (Deltapsi(m)) and reduced cytochrome c release and intrinsic pathway apoptosis activation. Further, FA modulated the expression of Peroxiredoxin, an anti-oxidative protein, and attenuated phosphorylation of ERK1/2 activated by Abeta oligomers.

yearjournalcountryeditionlanguage
2009-10-30
10.1080/10715760903214454http://hdl.handle.net/10447/54571