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RESEARCH PRODUCT
Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer.
Virginia Prieto-santiagoCelia CarrilloFrancisco J. BarbaSara R. Alonso-torreMaría Del Mar Caviasubject
Triple quadrupoleMultiple reaction monitoringMolecular biologyOligosaccharidesMass spectrometryMass SpectrometryAnalytical ChemistryStachyosechemistry.chemical_compoundAlimentosRaffinoseChromatography High Pressure Liquidchemistry.chemical_classificationBiología molecularChromatographySelected reaction monitoringExtraction (chemistry)Reproducibility of ResultsFabaceaeGeneral MedicineRepeatabilityOligosaccharideLegumesTriple quadrupole mass spectrometerchemistryFoodFood ScienceChromatography Liquiddescription
Raffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2022-01-01 | Food chemistry |