6533b86cfe1ef96bd12c80bb

RESEARCH PRODUCT

NEURONS PRODUCE FGF-2 AND VEGF SECRETE THEM AT LEST IN PART BY SHEDDING EXTRACELLULAR VESCICLES

Marco MineoChiara AlbertiItalia Di LiegroGiovanni SavettieriPatrizia ProiaGabriella Schiera

subject

Vascular Endothelial Growth Factor AFGF-2BiologyFibroblast growth factorchemistry.chemical_compoundsheddingNeurofilament ProteinsGlial Fibrillary Acidic ProteinExtracellularAnimalsSecretionRats WistarCells CulturedNeuronsVesicleIntegrin beta1Secretory VesiclesCell BiologyArticlesVEGFTransport proteinCell biologyRatsVascular endothelial growth factorVascular endothelial growth factor AProtein TransportMembrane proteinchemistryAstrocytesMolecular Medicineneurons vesicles fibroblastic growth factor-2 vascular endothelial growth factorCamptothecinFibroblast Growth Factor 2Extracellular Spaceextracellular vesicles

description

Abstract We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain β1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

yearjournalcountryeditionlanguage
2007-01-01
http://hdl.handle.net/10447/36887