6533b86cfe1ef96bd12c828b

RESEARCH PRODUCT

Adrenoceptor-mediated effects on calcium channel currents are antagonized by 5?-(N-ethyl)-carboxamido-adenosine in guinea-pig atrial cells

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description

In guinea-pig atrial myocytes, the effects of the adenosine analogue 5′-(N-ethyl)-carboxamido-adenosine (NECA) in the presence of isoprenaline (ISO) on Ca2+ channel activity were analyzed. Single Ca2+ channel currents were recorded from cell-attached patches by application of several hundred 100 ms depolarizing steps. Under control conditions, burstlike activity of channel openings during some depolarizing steps were followed by variably long periods of quiescence (blank sweeps). During superfusion with ISO (100 nmol/l), ensemble-averaged (mean) current was increased by about 150%. The underlying mechanism was found to be a significant increase in the channel availability, defined as the ratio of current-containing sweeps to the total number of sweeps. In addition, the ISO-induced reduction of blank sweeps was combined with slightly but not significantly higher values of the open probability in the current-containing sweeps. Open time and shut time histograms could be fitted by single and double exponential curves, respectively, which remained rather unaffected in the presence of ISO; accordingly, mean open time and mean shut time of the channel were not significantly changed by ISO. After the addition of NECA (1 μmol/l) in the presence of ISO, the ISO-induced increase in mean current was abolished. This effect of NECA on mean current was due to a reduction of the channel availability and a slight decrease in the open probability. The purinoceptor blocking agent 8-phenyltheophylline (10 μmol/l) antagonized the inhibitory action of NECA on the ISO-induced increase in Ca2+ channel activity. These results indicate that β-adrenoceptor-induced changes in Ca2+ channel activity are counteracted by stimulation of, presumably, A1 adenosine receptors. This may be explained by the fact that purinoceptors, beside their stimulating action on K+ channels, mediate an additional inhibitory effect on the adenylate cyclase system in the atrium. Subsequently, purinoceptor activation reverses the ISO-induced increase in Ca2+ channel activity due to a reduction in cAMP content leading to a decrease in the number of phosphorylated Ca2+ channels.