6533b86cfe1ef96bd12c8c7e
RESEARCH PRODUCT
Upregulation of antibody response to heat shock proteins and tissue antigens in an ocular ischemia model.
Nils BoehmDesiree R. DirkStephanie C. JoachimNorbert PfeifferMartin B. WaxFranz H. Grussubject
MalePathologymedicine.medical_specialtyIschemiaHSP27 Heat-Shock ProteinsProtein Array AnalysisVimentinBiologyAutoantigensDownregulation and upregulationAntigenRetinal DiseasesHeat shock proteinGlial Fibrillary Acidic ProteinmedicineAnimalsRats WistarEye ProteinsIntraocular PressureAutoantibodiesGlial fibrillary acidic proteinRetinal VesselsSpectrinMyelin Basic ProteinOptic Nervemedicine.diseaseAxonsRatsUp-RegulationMyelin-Associated GlycoproteinShock (circulatory)Immunoglobulin GReperfusion Injurybiology.proteinMyelin-Oligodendrocyte Glycoproteinmedicine.symptomAntibodyMyelin Proteinsdescription
PURPOSE. The aim of this study was to characterize the serum antibody reactivities occurring after ocular ischemia reperfusion. The time course of serum antibody responses was examined. METHODS. Wistar rats were exposed to transient ocular ischemia by elevating intraocular pressure to 130 mm Hg for 60 minutes. Axonal damage was evaluated on optic-nerve sections 2 and 4 weeks later. Blood samples collected before and several times after ischemia were used for antibody detection via customized protein microarrays. Different tissue antigens, including heat shock proteins (HSPs) and crystallins, were selected based on previous identification of antibody reactivities in studies on ischemic events or ophthalmic diseases associated with ischemia. Antibody reactivity was compared using multivariate statistical techniques. RESULTS. Significant axonal damage was observed 2 and 4 weeks after ocular ischemia (P 0.05). Animals showed certain immunoreactivities against antigens even before ischemia, whereas many reactivities increased afterward. Significantly different responses were detected 2, 3, and 4 weeks after ischemia (P 0.05). Antibody reactivity against actin, glial fibrillary acidic protein, HSP 27, vimentin, or spectrin continually increased. CONCLUSIONS. Ischemia induced by acute intraocular pressure elevation led to complex changes in antibody reactivities in sera of treated animals. Upregulation of serum autoantibodies, especially against heat shock and structural proteins, progressively increased throughout the 4-week follow-up period, whereas others such as ubiquitin decreased. The upregulation of anti‐HSP 27 antibodies might be an attempt to protect the tissue from ischemic damage. (Invest Ophthalmol Vis Sci. 2011;52:3468‐3474) DOI:10.1167/iovs.10-5763
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-02-12 | Investigative ophthalmologyvisual science |