6533b86cfe1ef96bd12c8d31
RESEARCH PRODUCT
Recent advances on aptamer-based biosensors to detection of platelet-derived growth factor.
Mohammad HasanzadehMaryam HejaziBehzad BaradaranMiguel De La GuardiaAhad MokhtarzadehJafar MosaferNasrin Razmisubject
Tumor angiogenesisModels MolecularPlatelet-derived growth factorProtein biomarkersComputer scienceAptamerBiomedical EngineeringBiophysicsBecaplermin02 engineering and technologyComputational biologyBiosensing Techniques01 natural scienceschemistry.chemical_compoundElectrochemistryAnimalsHumansTumor growth010401 analytical chemistryGeneral MedicineElectrochemical TechniquesProto-Oncogene Proteins c-sisAptamers Nucleotide021001 nanoscience & nanotechnology0104 chemical sciencesBiomarker (cell)Serum cytokinechemistryLuminescent MeasurementsNanoparticlesColorimetry0210 nano-technologyBiosensorBiotechnologydescription
Platelet-derived growth factor (PDGF-BB), a significant serum cytokine, is an important protein biomarker in diagnosis and recognition of cancer, which straightly rolled in proceeding of various cell transformations, including tumor growth and its development. Fibrosis, atherosclerosis are certain appalling diseases, which PDGF-BB is near to them. Generally, the expression amount of PDGF-BB increases in human life-threatening tumors serving as an indicator for tumor angiogenesis. Thus, identification and quantification of PDGF-BB in biomedical fields are particularly important. Affinity chromatography, immunohistochemical methods and enzyme-linked immunosorbent assay (ELISA), conventional methods for PDGF-BB detection, requiring high-cost and complicated instrumentation, take too much time and offer deficient sensitivity and selectivity, which restrict their usage in real applications. Hence, it is essential to design and build enhanced systems and platforms for the recognition and quantification of protein biomarkers. In the past few years, biosensors especially aptasensors have been received noticeable attention for the detection of PDGF-BB owing to their high sensitivity, selectivity, accuracy, fast response, and low cost. Since the role and importance of developing aptasensors in cancer diagnosis is undeniable. In this review, optical and electrochemical aptasensors, which have been applied by many researchers for PDGF-BB cancer biomarker detection, have been mentioned and merits and demerits of them have been explained and compared. Efforts related to design and development of aptamer-based biosensors using nanoparticles for sensitive and selective detection of PDGF-BB have been reviewed considering: Aptamer importance as recognition elements, principal, application and the recent improvements and developments of aptamer based optical and electrochemical methods. In addition, commercial biosensors and future perspectives for rapid and on-site detection of PDGF-BB have been summarized.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-08-01 | Biosensorsbioelectronics |