6533b86dfe1ef96bd12c9566

RESEARCH PRODUCT

The Mu1 transposable element of maize contains two promoter signals recognized by the Escherichia coli RNA polymerase.

Domenica Rita MassardoMario MottoF. MannaL. Del GiudiceKlaus Werner WolfPietro Alifano

subject

Transposable elementTranscription GeneticMolecular Sequence DataRestriction MappingBiologymedicine.disease_causeZea mayschemistry.chemical_compoundRNA polymeraseGeneticsmedicineEscherichia coliDirect repeatInsertion sequenceCloning MolecularPromoter Regions GeneticMolecular BiologyEscherichia coliGeneticsExpression vectorBase SequencePromoterDNA-Directed RNA PolymerasesGalactokinasechemistryDNA Transposable Elements

description

The galactokinase (GalK) expression plasmid vector system pKO-1 has been used to screen for promoter elements in the maize transposable element Mu1 that function in Escherichia coli. Two transcriptional start points, named S1 and S2, were identified, which are located in the two direct repeats of the transposable element. This paper demonstrates that sequence elements exist in a plant transposable element which function as prokaryotic promotors.

10.1007/bf00283025https://pubmed.ncbi.nlm.nih.gov/2172763