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RESEARCH PRODUCT
pH Feedback Lifecycles Programmed by Enzymatic Logic Gates Using Common Foods as Fuels
Xinlong FanAndreas WaltherAndreas Walthersubject
540 Chemistry and allied sciencesSystems ChemistryKinetics010402 general chemistry01 natural sciencesCatalysisenzymatic logic gatestemporal signalResearch Articleschemistry.chemical_classificationOxidase test010405 organic chemistryGeneral MedicineGeneral ChemistryBiocompatible material0104 chemical sciencesregulatory mechanismsCoupling (electronics)EnzymechemistrypH feedback540 ChemieLogic gatetransient hydrogelBiological systemResearch Articledescription
Abstract Artificial temporal signaling systems, which mimic living out‐of‐equilibrium conditions, have made large progress. However, systems programmed by enzymatic reaction networks in multicomponent and unknown environments, and using biocompatible components remain a challenge. Herein, we demonstrate an approach to program temporal pH signals by enzymatic logic gates. They are realized by an enzymatic disaccharide‐to‐monosaccharide‐to‐sugar acid reaction cascade catalyzed by two metabolic chains: invertase‐glucose oxidase and β‐galactosidase‐glucose oxidase, respectively. Lifetimes of the transient pH signal can be programmed from less than 15 min to more than 1 day. We study enzymatic kinetics of the reaction cascades and reveal the underlying regulatory mechanisms. Operating with all‐food grade chemicals and coupling to self‐regulating hydrogel, our system is quite robust to work in a complicated medium with unknown components and in a biocompatible fashion.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-04-07 | Angewandte Chemie International Edition |