6533b86dfe1ef96bd12c96b4
RESEARCH PRODUCT
S-type lectins occur also in invertebrates: high conservation of the carbohydrate recognition domain in the lectin genes from the marine sponge Geodia cydonium.
Martina HaasemannVera GamulinKarin PfeiferFalk FahrenholzHagen BrettingWerner E. G. M�llersubject
clone (Java method)GalectinsBlotting WesternMolecular Sequence DataBiochemistryChromatography AffinityMass SpectrometryLectinsAnimalsAmino Acid SequenceCloning MolecularPeptide sequenceGeneGalectinCloningbiologyBase SequencecDNA libraryLectinDNAbiology.organism_classificationPoriferaSuberites domunculaBiochemistrybiology.proteinElectrophoresis Polyacrylamide Geldescription
The marine sponge Geodia cydonium contains several lectins. The main component, called lectin-1, is composed of three to four identical subunits. The subunits of the lectins were cloned from a cDNA library; two clones were obtained. From the deduced aa sequence of one clone, LECT-1, a mol. wt of 15,313 Da is calculated; this value is in good agreement with mass spectrometric analysis of 15,453 +/- 25 Da. The sequence of another clone, LECT-2, was analysed and the aa sequence was deduced (15,433 Da). The two subunits have a framework sequence of 38 conserved aa which are characteristic for the carbohydrate-binding site of vertebrate S-type lectins. Clustering of lectin sequences of various species following their pairwise comparison establishes a dendrogram, which reveals that the sponge lectin could be considered as the ancestor for vertebrate S-type lectins.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-04-01 | Glycobiology |