6533b86dfe1ef96bd12c96f0
RESEARCH PRODUCT
Enantiomeric separation of amphetamine and related compounds by liquid chromatography using precolumn derivatization witho-phthaldialdehyde
Jorge Verdú-andrésPilar Campí Ns-falcóRosa Herráez-hernándezsubject
Detection limitChromatographyOrganic ChemistryClinical BiochemistryEnantioselective synthesisDiastereomerReversed-phase chromatographyBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundchemistrymedicineEnantiomerDerivatizationPhenylpropanolaminemedicine.drugdescription
The utility of the derivatization reagento-phthaldialdehyde (OPA) in combination with the chiral thiol N-acetyl-L-cysteine (NAC) for the enantioselective analysis of primary amphetamines and structurally related substances has been investigated. The diastereomers obtained have been chromatographed on an achiral, reversed phase C18 column (125 mm×4 mm i.d., 5 μm), and detected fluorimetrically. The influence of parameters affecting the chromatographic separation as well as the derivatization reaction has been optimized, using amphetamine, norephedrine, norepinephrine and 3,4-methylenedioxyamphetamine (MDA) as model compounds. On the basis of the results obtained, possible conditions for the enantiomeric analysis of these substances are described. The selected conditions have been used successfully for the enantioselective quantitation of the compounds tested within 0.5–10.0 μg mL−1 concentration interval (each enantiomer), the limits of detection being 25–50 ng mL−1. Examples of application to biological samples are also given.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-11-01 | Chromatographia |