6533b86dfe1ef96bd12c974d
RESEARCH PRODUCT
Interaction of sulphate and chloride with cobalt(II)-carbonic anhydrase
José M. MoratalJesus SalgadoAntonio DonaireMaria-josé Martinez-ferrersubject
Magnetic Resonance SpectroscopyCarbonic anhydrase IICoordination numberInorganic chemistryIon chromatographychemistry.chemical_elementBiochemistryChlorideAdductInorganic ChemistryChloridesCarbonic anhydrasemedicineCarbonic Anhydrase InhibitorsCarbonic AnhydrasesbiologySulfatesChemistryOsmolar ConcentrationTemperatureCobaltHydrogen-Ion ConcentrationSpectrophotometrybiology.proteinProton NMRCobaltmedicine.drugdescription
The interaction between Cobalt(II)-Bovine Carbonic Anhydrase II and the inhibitors sulphate and chloride have been investigated through 1H NMR and electronic absorption spectroscopies. Both inhibitors bind to the metal ion forming a 1:1 adduct and the corresponding affinity constants have been determined. These inhibitors interact weakly with CoBCA II and this interaction only occurs at low pH values. The T1 values of the meta-like protons of the coordinated histidines have been measured. The coordination number of the metal ion in the adducts is discussed on the basis of temperature dependence of the isotropic shifts, T1, and molar absorbance values.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-11-01 | Journal of Inorganic Biochemistry |