6533b86dfe1ef96bd12ca0c6

RESEARCH PRODUCT

Glucose uptake in germinating Aspergillus nidulans conidia: involvement of the creA and sorA genes

Pilar MiróDaniel RamónDaniel RamónLuisa VenturaAndrew Maccabe

subject

biologyGlucose uptakeGenes FungalMutantFungal geneticsCatabolite repressionBiological Transport ActiveRepressorCarbohydrate metabolismbiology.organism_classificationMicrobiologyAspergillus nidulansConidiumFungal ProteinsRepressor ProteinsKineticsGlucoseBiochemistryDrug Resistance FungalAspergillus nidulansMutationSorbose

description

d-Glucose uptake in germinating wild-typeAspergillus nidulansconidia is an energy-requiring process mediated by at least two transport systems of differing affinities for glucose: a low-affinity system (Km∼1·4 mM) and a high-affinity system (Km∼16 μM). The low-affinity system is inducible by glucose; the high-affinity system is subject to glucose repression effected by the carbon catabolite repressor CreA and is absent insorA3mutant conidia, which exhibit resistance tol-sorbose toxicity. An intermediate-affinity system (Km∼400 μM) is present insorA3conidia germinating in derepressing conditions.creAderepressed mutants show enhanced sensitivity tol-sorbose. The high-affinity uptake system appears to be responsible for the uptake of this toxic sugar.

yearjournalcountryeditionlanguage
2003-08-01Microbiology
https://doi.org/10.1099/mic.0.26349-0