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RESEARCH PRODUCT

Phytochemical, ecological and antioxidant evaluation of wild Sicilian thyme: Thymbra capitata (L.) Cav.

Salvatore La BellaTeresa TuttolomondoGiuseppe BonsangueClaudio LetoAntonella SaijaEdoardo NapoliMario LicataLaura SiracusaMaria Cristina GennaroGiuseppe VirgaGiuseppe RubertoDomenico TrombettaAntonio Speciale

subject

Antioxidantmedicine.medical_treatmentPhytochemicalsBioengineering01 natural sciencesBiochemistryAntioxidantslaw.inventionchemistry.chemical_compoundfoodlawBotanyAntioxidant activity Bioagronomic characterization Essential oil; Lamiaceae Polyphenols Thyme (Thymbra capitata (L.) Cav.)Oils VolatilemedicineCarvacrolFood scienceMolecular BiologyEssential oilLamiaceaeMolecular Structurebiology010405 organic chemistryRosmarinic acidGeneral ChemistryGeneral Medicinebiology.organism_classificationfood.foodSettore AGR/02 - Agronomia E Coltivazioni Erbacee0104 chemical sciences010404 medicinal & biomolecular chemistryPhytochemicalchemistryPolyphenolThyme (Thymbra capitata (L.) CAV.) Lamiaceae Bioagronomic characterization Essential oil Polyphenols Antioxidant activities.MonoterpenesCymenesMolecular MedicineThymus capitatusLamiaceaeThyme (Thymbra capitata (L.) Cav.); Lamiaceae; Bioagronomic characterization; Essential oil; Polyphenols; Antioxidant activity.

description

In a broad survey conducted throughout the Sicily region, 45 different sites were identified where thyme grows wild. All the biotypes collected were classified as Thymbra capitata (L.) Cav. (syn. Thymus capitatus (L.) Hoffmanns. & Link). Cluster analysis based on the main morphological characteristics of the plant led to the division of the biotypes into 3 major groups. All samples were analyzed for their secondary phytochemical metabolites identified in the extracts and the essential oils. LC-UV-DAD/ESI-MS and GC-FID/GC-MS have been applied to characterize the extracts and the essential oils, respectively. In the extracts, 15 flavonoid derivatives with taxifolin-di-O-glucoside and thymusin as main components, and 2 organic acids, with a large predominance of rosmarinic acid, were identified. On the whole 37 compounds were fully characterized in the essential oils, carvacrol was identified as the main component with an average value of 73.93%. The total phenol content and the antioxidant activity of all phytochemical complexes were determined with the Folin-Ciocalteu (FC) assay, the UV radiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of superoxide radical (O2∙-).

10.1002/cbdv.201600072http://hdl.handle.net/11570/3087593