6533b86dfe1ef96bd12ca8a3
RESEARCH PRODUCT
Action de la chlorhexidine sur l'expression de la virulence de
G. MathieuMaurice BensoussanMarc SautourA. DelcourtCharles Divièssubject
biologymedicine.drug_classChlorhexidineProteolytic enzymesbiology.organism_classificationYeastMicrobiologyMinimum inhibitory concentrationAntisepticBiochemistryCaseinmedicinebiology.proteinBovine serum albuminCandida albicansEcology Evolution Behavior and Systematicsmedicine.drugdescription
Abstract Candida albicans is an opportunistic yeast. Its pathogenicity is linked to the susceptibility of the host surface as well as to particular factors of the strain: adhesion, filamentous growth and secretion of proteolytic enzymes. Chlorhexidine digluconate is an antiseptic with fungicidal properties. The action of the antiseptic on the growth of the yeast shows a minimal inhibitory concentration (MIC) at 50 μg·mL−1 and a minimal fungicidal concentration (MFC) at 100 μg·mL−1. The consequences of antiseptic treatment are studied using two indicators of pathogenicity: filamentation and the secretion of acid proteinase. Concerning the morphological indicator, a complete inhibition of filamentation for antiseptic concentration far below the MIC is observed; concerning the biochemical indicator, the results show a maintenance of proteolytic activity for concentration equal to the MIC and a disappearance of this activity between the MIC and the MFC. Concerning the proteolytic activity, Skim Milk (Difco) was substituted for bovine serum albumin with success. This mixture of casein allows to use a cheaper and more easily available substrate.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-07-01 | Cryptogamie Mycologie |