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RESEARCH PRODUCT
Visual Contrast Modulates Operant Learning Responses in Larval Zebrafish.
Wenbin YangWenbin YangYutong MengYutong MengDanyang LiDanyang LiQuan WenQuan WenQuan Wensubject
visionCognitive Neuroscienceved/biology.organism_classification_rank.specieseducationautomated image analysisBehavioral neurosciencelcsh:RC321-57103 medical and health sciences0302 clinical medicinebiology.animalZebrafish larvaehigh-throughput imagingOperant conditioningModel organismlcsh:Neurosciences. Biological psychiatry. Neuropsychiatry030304 developmental biologyOriginal Research0303 health scienceslearningbiologyved/biologyRepertoirefungiVertebrateExtinction (psychology)Neuropsychology and Physiological PsychologyLearning curvezebrafish larvaebehavioral neuroscienceNeuroscience030217 neurology & neurosurgeryNeurosciencedescription
The larval zebrafish is a promising vertebrate model organism to study neural mechanisms underlying learning and memory due to its small brain and rich behavioral repertoire. Here, we report on a high-throughput operant conditioning system for zebrafish larvae, which can simultaneously train 12 fish to associate a visual conditioned pattern with electroshocks. We find that the learning responses can be enhanced by the visual contrast, not the spatial features of the conditioned patterns, highlighted by several behavioral metrics. By further characterizing the learning curves as well as memory extinction, we demonstrate that the percentage of learners and the memory length increase as the conditioned pattern becomes darker. Finally, little difference in operant learning responses was found between AB wild-type fish and elavl3:H2B-GCaMP6f transgenic fish.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-09-03 | Frontiers in behavioral neuroscience |