6533b86dfe1ef96bd12cab23
RESEARCH PRODUCT
Biomimetic strategy towards gelatin coatings on PET. Effect of protocol on coating stability and cell-interactive properties
Filip De VosElena Diana GiolElena Diana GiolC. J. KirkpatrickC. J. KirkpatrickSandra Van VlierbergheSandra Van VlierberghePeter DubruelRonald E. UngerKen Kersemanssubject
food.ingredientBiocompatibilityCellBiomedical Engineering02 engineering and technologyengineering.material010402 general chemistry01 natural sciencesGelatinfoodCoatingSmooth muscleBiomimeticsmedicineGeneral Materials ScienceChemistryPolyethylene TerephthalatesGeneral ChemistryGeneral Medicine021001 nanoscience & nanotechnology0104 chemical sciencesPolyestermedicine.anatomical_structureengineeringSurface modificationGelatin0210 nano-technologyVascular graftBiomedical engineeringSURFACE MODIFICATION; CHEMISTRY; POLYESTER; ADHESION; FUNCTIONALIZATION; BIOCOMPATIBILITY; IMMOBILIZATION; PROLIFERATION; COMPATIBILITY; BIOMATERIALSdescription
Gelatin-modified poly(ethylene terephthalate) (PET) surfaces have been previously realized via an intermediate dopamine coating procedure that resulted in surfaces with superior haemocompatibility compared to unfunctionalized PET. The present study addresses the biocompatibility assessment of these coated PET surfaces. In this context, the stability of the gelatin coating upon exposure to physiological conditions and its cell-interactive properties were investigated. The proposed gelatin–dopamine-PET surfaces showed an increased protein coating stability up to 24 days and promoted the attachment and spreading of both endothelial cells (ECs) and smooth muscle cells (SMCs). In parallel, physisorbed gelatin coatings exhibited similar cell-interactive properties, albeit temporarily, as the coating delaminated within 1 week after cell seeding. Furthermore, no or only minimal immunogenic or inflammatory responses were observed during in vitro cytotoxicity and endotoxicity assessment for all gelatin-modified PET surfaces evaluated. Overall, the combined enhanced biocompatibility reported herein together with the previously proven haemocompatibility show the potential of the gelatin–dopamine-PET surfaces to function as vascular graft candidates.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-02-28 |