6533b86efe1ef96bd12cac5b

RESEARCH PRODUCT

Specific inflammatory response of Anemonia sulcata (Cnidaria) after bacterial injection causes tissue reaction and enzymatic activity alteration

Maria Rosa TrapaniDaniela ParrinelloMatteo CammarataMaria Giovanna ParisiGigliola BenenatiFranco PallaMaria Antonietta Sanfratello

subject

0301 basic medicinemedicine.medical_treatmentPhosphatasemedicine.disease_causeEsteraseMicrobiology03 medical and health sciences0302 clinical medicineEscherichia colimedicineAnimals030212 general & internal medicineEscherichia coliInflammation Anemonia sulcata Cnidaria Bacterial injection Esterases PhosphatasesVibrio alginolyticusEcology Evolution Behavior and SystematicsInflammationchemistry.chemical_classificationVibrio alginolyticusProteasebiologyFibrinolysisEsterasesFibrinogenAlkaline Phosphatasebiology.organism_classificationPhosphoric Monoester HydrolasesSea Anemones030104 developmental biologyEnzymechemistryHost-Pathogen InteractionsGelatinAlkaline phosphataseElectrophoresis Polyacrylamide GelBacteriaDensitometryPeptide Hydrolases

description

The evolution of multicellular organisms was marked by adaptations to protect against pathogens. The mechanisms for discriminating the ’’self’’ from ’’non-self” have evolved into a long history of cellular and molecular strategies, from damage repair to the co-evolution of host-pathogen interactions. We investigated the inflammatory response in Anemonia sulcata (Cnidaria: Anthozoa) following injection of substances that varied in type and dimension, and observed clear, strong and specific reactions, especially after injection of Escherichia coli and Vibrio alginolyticus. Moreover, we analyzed enzymatic activity of protease, phosphatase and esterase, showing how the injection of different bacterial strains alters the expression of these enzymes and suggesting a correlation between the appearance of the inflammatory reaction and the modification of enzymatic activities. Our study shows for the first time, a specific reaction and enzymatic responses following injection of bacteria in a cnidarian.

yearjournalcountryeditionlanguage
2015-01-31Journal of Invertebrate Pathology
https://doi.org/10.1016/j.jip.2016.01.010