6533b86efe1ef96bd12cb40f

RESEARCH PRODUCT

4-Diazopyrazole Derivatives as Potential New Antibiofilm Agents

Maria Grazia CusimanoBenedetta MaggioGiuseppe DaidoneDomenico SchillaciRaffa DemetrioMaria Valeria RaimondiStella Cascioferro

subject

StaphylococcusGerm tubeMicrobiologychemistry.chemical_compoundCandida albicansDrug DiscoveryPharmacology (medical)Crystal violetCandida albicansPharmacologyTube formationAza CompoundsMolecular StructurebiologyStaphylococcus biofilms Candida Albicans biofilms Antibiofilms activity DiazopyrazolesBiofilmBiological activityGeneral Medicinebiology.organism_classificationAntimicrobialSettore CHIM/08 - Chimica FarmaceuticaCorpus albicansInfectious DiseasesOncologychemistryBiofilmsPyrazoles

description

<i>Background:</i> The recognition that chronic infections and infections associated with medical devices are biofilm related has been the impulse for investigating the antibiofilm properties of some diazopyrazoles biologically active as antimicrobials. <i>Methods:</i> The susceptibility of staphylococcal biofilms was determined at concentrations ranging from 25 to 1.5 µg/ml using crystal violet and methylthiazotetrazolium (MTT) staining. In the case of <i>Candida albicans,</i> we first assessed the anti-germ tube formation effect of 4-NO<sub>2</sub> (compound 1c) and then we evaluated its antibiofilm activity at concentrations ranging from 100 to 6.2 µg/ml. <i>Results:</i> All the compounds were active against reference staphylococcal biofilm strains at the maximum tested concentration of 25 µg/ml; despite its remarkable activity as germ tube inhibitor, compound 1c showed weak activity against <i>C. albicans</i> biofilms at 100 µg/ml. <i>Conclusion:</i> 4-Diazopyrazoles represent an interesting source of antimicrobial compounds in the development of new antibiofilm agents.

yearjournalcountryeditionlanguage
2008-03-04
10.1159/000159271http://hdl.handle.net/10447/39025