Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity.

6533b86efe1ef96bd12cb461

RESEARCH PRODUCT

Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity.

Corine Zotti Caroline Kunz Agnes Cimerman Regine Vedel Stéphane G. Rolland Christophe Bruel Benoît Poinssot Martine Boccara Elisabeth Moreau Alain Pugin Elodie Vandelle

subject

Hypersensitive response hypersensitive response Physiology [SDV]Life Sciences [q-bio]colonisation Mutant Arabidopsis Conidiation Plant Science Microbiology Insertional mutagenesis Fungal Proteins 03 medical and health sciences Plasmid Gene Expression Regulation Fungal Onions Botrytis cinerea; endopolygalacturonase BcPG1; hypersensitive response Vitis mutant oxidative burst Pathogen Gene 030304 developmental biology Botrytis cinerea Phaseolus 0303 health sciences oxalate endopolygalacturonase BcPG1 biology 030306 microbiology Oxalic Acid fungi food and beverages Hydrogen-Ion Concentration biology.organism_classification Immunity Innate nonpathogenic mutant Enzymes Plant Leaves Mutagenesis Insertional nonaspartyl acid protease ACP1 Mutation botrytis cinerea;endopolygalacturonase BcPG1;hypersensitive response;nonaspartyl acid protease ACP1;nonpathogenic mutant;oxalate;oxidative burst Botrytis botrytis cinerea Reactive Oxygen Species

description

International audience; Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species.Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized.Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine ( Vitis vinifera ) suspension cells, and the mutant–plant interaction resulted in the formation of hypersensitive response-like necrosis. Genetic segregation analyses revealed that the pathogenicity phenotype was linked to a single locus, but showed that the mutated gene was not tagged by the plasmid pAN7-1.Mutant A336 is the first oxalate-deficient mutant to be described in B. cinerea and it differs from all the nonpathogenic B. cinerea mutants described to date.

year	journal	country	edition	language
2006-01-01	The New phytologist

10.1111/j.1469-8137.2006.01682.x https://pubmed.ncbi.nlm.nih.gov/16626475