6533b86efe1ef96bd12cb479
RESEARCH PRODUCT
Cytocidal effects of Escherichia coli hemolysin on human T lymphocytes.
C. KlasSucharit BhakdiB. SchultheisDaniel JonasPeter H. Krammersubject
Programmed cell deathCell Membrane PermeabilityTime FactorsDNA damageT-LymphocytesImmunologyBiologyIn Vitro Techniquesmedicine.disease_causeHemolysin ProteinsLymphocyte ActivationMicrobiologyMicrobiologyHemolysin ProteinsAdenosine TriphosphatemedicineEscherichia coliCytotoxic T cellHumansEscherichia coliCell DeathDose-Response Relationship DrugHemolysinT lymphocyteDNAInfectious DiseasesDNA fragmentationParasitologyResearch ArticleDNA Damagedescription
Escherichia coli hemolysin is the prototype of a large family of pore-forming toxins produced by gram-negative organisms. Besides its known cytotoxic activities against granulocytes, monocytes, endothelial cells, and renal epithelial cells, we now demonstrate that the toxin potently kills human T lymphocytes. Evidence based on different and independent approaches indicates that lymphocidal activity is due to formation of transmembrane pores. Additionally, cells prestimulated with phytohemagglutinin respond to low doses of E. coli hemolysin with DNA fragmentation similar to that observed in cells undergoing programmed cell death. Kinetic considerations lead us to conclude that DNA degradation may, however, represent an epiphenomenon. Killing of T cells is another means through which E. coli hemolysin could directly impair host defense.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-05-01 |