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RESEARCH PRODUCT

Improved detection of alkaloids in crude extracts applying capillary electrophoresis with field amplified sample injection

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Abstract A simple and effective method for the sensitive detection of alkaloids in crude plant extracts applying capillary electrophoresis with field amplified sample injection (FASI) is described. This method was compared with normal pressure injection for the determination of alkaloids in methanolic extracts from roots of Berberis vulgaris L. (Berberidaceae) and Hydrastis canadensis L. (Ranunculaceae) using a 1:1 mixture of 200 mM ammonium acetate at pH 3.1 and methanol. By introducing a short plug of 70% methanol (v/v) before electrokinetic injection with 16 kV for 8 s the concentration sensitivity was 1000-times higher compared to hydrodynamic injection for 1 s. No difference between both injection methods for selectivity and resolution of the obtained electropherograms was found. The influence of voltage and injection time on the introduced sample amount was investigated using a mixture of berberine and chelidonine as model substances.