6533b86efe1ef96bd12cbca5
RESEARCH PRODUCT
Coupling of proton translocation through ATPase incorporated into supported lipid bilayers to an electrochemical process
Helmut RingsdorfC. HampelN. BunjesPeter GräberRenate L. C. NaumannAlfred JonczykWolfgang Knollsubject
biologyChemistryATPaseBilayerBiophysicsAnalytical chemistryChronoamperometryElectrochemistryElectrodeElectrochemistrybiology.proteinBiophysicsLipid bilayer phase behaviorPhysical and Theoretical ChemistryLipid bilayerElectrochemical gradientdescription
Abstract H+-ATPase is incorporated into solid-supported lipid bilayers separated from the gold support by a peptide spacer. The translocation of protons across the lipid film to the inner side is coupled to the discharge of protons at the gold surface. The overall process is investigated by square wave voltammetry (SWV) and double potential-pulse chronoamperometry (CA). As a result, the formation of a proton gradient is monitored by SWV whereas currents measured by CA monitor the stationary state when the enzyme activity is directly coupled to the charge transfer at the electrode. These currents markedly depend on the number of ATPases present in the bilayer.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-05-01 | Bioelectrochemistry and Bioenergetics |