6533b86efe1ef96bd12cbdf0

RESEARCH PRODUCT

In Situ, Light-Guided Axon Growth on Biomaterials via Photoactivatable Laminin Peptidomimetic IK(HANBP)VAV

Aleeza FarrukhAleeza FarrukhShifang ZhaoShifang ZhaoMarcelo SaliernoAránzazu Del CampoAránzazu Del CampoAtria KavyanifarAdolfo CavaliéJulieta I. Paez

subject

0301 basic medicineIn situMaterials scienceNeuritePeptidomimeticNeuronal OutgrowthPeptideINGENIERÍAS Y TECNOLOGÍAS02 engineering and technologyBiotecnología Industrial03 medical and health sciencesMiceCoated Materials BiocompatibleNeural Stem CellsDIRECTIONAL NEURONAL GROWTHLamininIKVAVNeuritesAnimalsGeneral Materials Sciencechemistry.chemical_classificationbiologyPHOTO-TRIGGERED CELL ADHESIONBioproductos Biomateriales Bioplásticos Biocombustibles Bioderivados etc.Hydrogels021001 nanoscience & nanotechnologyNeural stem cellPeptide FragmentsLAMININ PEPTIDOMIMETICS030104 developmental biologychemistryCell cultureSelf-healing hydrogelsbiology.proteinBiophysicsLamininPeptidomimetics0210 nano-technology

description

The ability to guide the growth of neurites is relevant for reconstructing neural networks and for nerve tissue regeneration. Here, a biofunctional hydrogel that allows light-based directional control of axon growth in situ is presented. The gel is covalently modified with a photoactivatable derivative of the short laminin peptidomimetic IKVAV. This adhesive peptide contains the photoremovable group 2-(4′-amino-4-nitro-[1,1′-biphenyl]-3-yl)propan-1-ol (HANBP) on the Lys rest that inhibits its activity. The modified peptide is highly soluble in water and can be simply conjugated to -COOH containing hydrogels via its terminal -NH 2 group. Light exposure allows presentation of the IKVAV adhesive motif on a soft hydrogel at desired concentration and at defined position and time point. The photoactivated gel supports neurite outgrowth in embryonic neural progenitor cells culture and allows site-selective guidance of neurites extension. In situ exposure of cell cultures using a scanning laser allows outgrowth of neurites in desired pathways. Fil: Farrukh, Aleeza. Leibniz Institute for New Materials; Alemania. Max Planck Graduate Center; Alemania Fil: Zhao, Shifang. Universitat Saarland; Alemania. Leibniz Institute for New Materials; Alemania Fil: Paez, Julieta Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Leibniz Institute for New Materials; Alemania Fil: Kavyanifar, Atria. Johannes Gutenberg Universitat Mainz; Alemania Fil: Salierno, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Universidad de Buenos Aires; Argentina. Johannes Gutenberg Universitat Mainz; Alemania Fil: Cavalié, Adolfo. Universitat Saarland; Alemania Fil: del Campo, Aránzazu. Universitat Saarland; Alemania. Leibniz Institute for New Materials; Alemania

yearjournalcountryeditionlanguage
2018-01-01ACS Applied Materials & Interfaces
10.1021/acsami.8b15517http://dx.doi.org/10.1021/acsami.8b15517