6533b86efe1ef96bd12cbeaf
RESEARCH PRODUCT
Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria.
Sergi FerrerLucä±ìa BlascoIsabel Pardosubject
In situDNA BacterialMolecular Probe TechniquesWineBiologyMicrobiologyDNA Ribosomalchemistry.chemical_compoundGeneticsLactic AcidPediococcusMolecular BiologyIn Situ Hybridization FluorescenceFluorescent DyesWineBase SequenceOligonucleotidefood and beverages16S ribosomal RNAbiology.organism_classificationFluorescenceMolecular biologyLactic acidLactobacillusBiochemistrychemistryFermentationIdentification (biology)Oligonucleotide ProbesBacteriaLeuconostocdescription
A rapid method for the identification of lactic acid bacteria (LAB) from wine has been developed. This method is based on fluorescence in situ hybridisation (FISH), using fluorescent oligonucleotide probes, homologous to 16S rDNA of those species of LAB commonly found in wines. The protocol for the specific detection of these bacteria was established through the hybridisation of 36 reference strains. The specificity of the probes was evaluated by using pure cultures. Probes were used to identify species in different wines, making it evident that direct identification and quantification from natural samples without culturing is also possible. The results show that FISH is a promising technique for the rapid identification of LAB, allowing positive identification in a few hours (4–16 h).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-08-01 | FEMS microbiology letters |