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RESEARCH PRODUCT
Ion-induced fluorescence imaging of endosomes
Frank WattXiao ChenAndrew A. BettiolM. ZhaohongVarpu MarjomäkiHarry J. WhitlowRen MinqinRattanaporn NoraratCe-belle Chensubject
Nuclear and High Energy PhysicsFluorescence-lifetime imaging microscopyta114biologyEndosomeChemistryResolution (electron density)ta1182Analytical chemistryFluorescenceAutofluorescenceCytoplasmMicroscopybiology.proteinBiophysicsEpidermal growth factor receptorInstrumentationdescription
Abstract Imaging laboratories at Jyvaskyla and Singapore are collaborating on the development of fluorescence imaging of cytoplasmic endosomes using a combination of proton induced fluorescence (PIF) with direct Scanning Transmission Ion Microscopy (direct-STIM) for sub-cellular structural imaging. A549 lung carcinoma cells were cultivated and stained for epidermal growth factor receptor (EGFR) and receptor α2β1 integrin. In this paper, we demonstrate that cells can be imaged at sub-150 nm resolution using the PIF technique. In addition, the same target cell was imaged at 50 and 25 nm resolution by using proton and He-STIM, respectively. The combination of both techniques offer a powerful tool to improve fluorescence imaging beyond optical diffraction limits.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-07-01 | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms |