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RESEARCH PRODUCT
Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas
Silvio GianinazziGwã©naã«lle Bestel-correRamu Subramania SaravananMarc St-arnaudGhislaine RecorbetBastien FontaineMarc DieuEliane Dumas-gaudotMartine RaesBenoã®t Valotsubject
0106 biological sciencesProteomeFungusProteomicsPlant Roots01 natural sciencesApplied Microbiology and BiotechnologyMicrobiologyMass SpectrometryMicrobiologyFungal Proteins03 medical and health sciencesSymbiosisMycorrhizaeElectrophoresis Gel Two-DimensionalMycorrhizaGlomeromycota[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyComputingMilieux_MISCELLANEOUSGel electrophoresis0303 health sciencesFungal proteinEcologybiology030306 microbiologyfungibiology.organism_classificationDaucus carotaArbuscular mycorrhiza[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology010606 plant biology & botanyDaucus carotadescription
To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase beta mitochondrial precursor. The possible fungal origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-06-01 |