6533b86efe1ef96bd12cc8f8
RESEARCH PRODUCT
Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defect repair.
Valerio BrucatoGioacchino ConoscentiViviana CostaGianluca GiavaresiRossella Di FalcoFrancesco Carfì PaviaValeria CarinaDaniele BellaviaLavinia RaimondiVincenzo La CarrubbaIlenia VitranoAngela De Lucasubject
0106 biological sciences0301 basic medicine3D cultureScaffoldCellular differentiationBioreactorBioengineeringBone tissue01 natural sciencesApplied Microbiology and BiotechnologyBone and BonesCell Line03 medical and health sciencesBioreactorsTissue engineeringPolylactic Acid-Polyglycolic Acid CopolymerPoly-L-lactic-acid/nano-hydroxyapatiteOsteogenesis010608 biotechnologyOsteogenic differentiation w/o growth factorsmedicineHumansBone regenerationCell ProliferationComposite scaffoldSettore ING-IND/24 - Principi Di Ingegneria ChimicaTissue EngineeringTissue ScaffoldsChemistryMesenchymal stem cell3D culture; Bioreactor; Composite scaffold; Osteogenic differentiation w/o growth factors; Poly-L-lactic-acid/nano-hydroxyapatite; Bioreactors; Bone and Bones; Cell Differentiation; Cell Line; Cell Proliferation; Durapatite; Humans; Mesenchymal Stem Cells; Osteogenesis; Polylactic Acid-Polyglycolic Acid Copolymer; Tissue Engineering; Tissue ScaffoldsSettore ING-IND/34 - Bioingegneria IndustrialeCell DifferentiationMesenchymal Stem CellsCell biologyRUNX2030104 developmental biologymedicine.anatomical_structureDurapatiteCell cultureBiotechnologydescription
Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic (d3D) 3D culture conditions at 7 and 21 days. The colonization rate of hMSCs and osteogenic differentiation were amplified by d3D when physical stimulation was provided by a perfusion bioreactor. Increase in dsDNA content (p < 0.0005), up- regulation of RUNX2, ALPL, SPP1 (p < 0.0005) and SOX9 (p < 0.005) gene expression, and more calcium nodule forma- tion (p < 0.0005) were observed in d3D cultures in comparison to s3D ones over time. Dynamic 3D culture, mimicking the mechanical signals of bone environment, improved significantly osteogenic differentiation of hMSCs on PLLA/nHA scaffold, without the addition of growth factors, confirming this composite scaffold suitable for bone regeneration
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-01 | Journal of bioscience and bioengineering |