6533b86efe1ef96bd12cc968
RESEARCH PRODUCT
Glioblastoma-associated circulating monocytes and the release of epidermal growth factor.
Axel PerneczkyGeorg FriesOliver Kempskisubject
MaleEpidermal Growth Factorbusiness.industryBrain NeoplasmsGrowth factormedicine.medical_treatmentMonocyteMiddle Agedmedicine.diseaseImmunohistochemistryMonocytesmedicine.anatomical_structureGrowth factor receptorEpidermal growth factorCell cultureGliomaImmunologyCancer researchmedicineInterleukin 19MacrophageHumansFemalebusinessGlioblastomadescription
✓ Monocytes/macrophages frequently infiltrate malignant gliomas and play a central role in the tumor-associated immune response as they process tumor antigen and present it to T-lymphocytes. Findings have accumulated that peripheral blood monocytes leaving the cerebral circulation become microglial cells and vice versa and that monocytes/macrophages may stimulate malignant tumor growth by some unknown mechanism. Most malignant gliomas express growth factor receptors, for example epidermal growth factor receptor (EGFR). The aim of this study was to determine whether peripheral blood monocytes of glioma patients release EGF, the appropriate ligand of gliomacell membrane-bound EGFR. Long-term cultured peripheral blood monocytes from 14 patients with malignant gliomas were compared to those from 12 controls (seven with nontumorous disease and five healthy individuals). Using an enzyme-linked immunosorbent assay for EGF, the EGF content of cell culture supernatants was determined at Days 7, 21, and 100 of culture. The EGF content (mean ± standard error) of supernatants was 5.9 ± 0.2 pg/ml/103 glioma monocytes versus 1.3 ± 0.1 pg/ml/103 control monocytes at Day 7 of culture, 22.9 ± 0.8 pg/ml/103 glioma monocytes versus 1.8 ± 0.9 pg/ml/103 control monocytes at Day 21 of culture, and 23.4 ± 0.7 pg/ml/103 glioma monocytes, and below detection levels for control monocytes at Day 100 of culture. Steroid treatment of glioma patients did not influence the EGF release of cultured monocytes. These data indicate that glioblastoma-associated peripheral blood monocytes may be distinct from those of healthy individuals. Moreover, this study indicates that subtypes of glioma-associated peripheral blood monocytes may support immunosuppression and promote growth of malignant glioma by releasing unusually high amounts of EGF.
year | journal | country | edition | language |
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1996-10-01 | Journal of neurosurgery |