6533b86efe1ef96bd12ccaf8

RESEARCH PRODUCT

Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

María Eugenia Suárez-ojedaDario PrestiGabriela Montiel-jarilloGiorgio Mannina

subject

0106 biological sciencesEnvironmental EngineeringPHABioengineeringSequencing batch reactorWastewater treatment010501 environmental sciencesWastewater01 natural sciencesBioplasticPolyhydroxyalkanoateschemistry.chemical_compoundBioreactors010608 biotechnologyBiomassWaste Management and DisposalEffluent0105 earth and related environmental sciencesSettore ICAR/03 - Ingegneria Sanitaria-AmbientaleRenewable Energy Sustainability and the EnvironmentChemistryPolyhydroxyalkanoatesExtraction (chemistry)Water resource recoveryGeneral MedicinePulp and paper industryLauric acidBioplasticWastewaterFermentationFermentationPlastics

description

Abstract A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.

yearjournalcountryeditionlanguage
2019-06-01
10.1016/j.biortech.2019.03.037http://hdl.handle.net/10447/367164