6533b86ffe1ef96bd12cd1b7
RESEARCH PRODUCT
Comparison of the interaction of cobalt bovine carbonic anhydrase II with acetazolamide and methazolamide and the reaction of apoenzyme with cobalt(II) complexes of acetazolamide and methazolamide: Spectrophotometric study
Gloria AlzuetJoaquín BorrásConsuelo BorrasSilvia Rubiosubject
chemistry.chemical_classificationbiologyCarbonic anhydrase IIInorganic chemistrychemistry.chemical_elementBioinorganic chemistryBiochemistryEnzymechemistryCarbonic anhydrasemedicineMetalloproteinbiology.proteinMethazolamideAcetazolamideMolecular BiologyCobaltNuclear chemistrymedicine.drugdescription
The metalloenzyme carbonic anhydrase (CA) is an attractive choice for a research-based bioinorganic laboratory course. In this project the interaction of cobalt bovine carbonic anhydrase II (CoBCAII) with acetazolamide and methazolamide and the reaction of apoenzyme with cobalt(II) complexes of acetazolamide and methazolamide is studied by UV-visible spectroscopy. Prior to this spectroscopic study students are given native BCAII, and they prepare apoBCAII and CoBCAII. A major aim is to provide experience in handling metalloproteins and in the study of metal complexes-protein interactions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-01-01 | Biochemistry and Molecular Biology Education |