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RESEARCH PRODUCT

Additional file 3: of Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses

Venla MustonenJenni KesäniemiAnton LavrinienkoEugene TukalenkoTapio MappesPhillip WattsJaana Jurvansuu

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Etoposide induces apoptosis in bank vole fibroblasts. We treated the cells with DMSO or 20 μM of etoposide for 24 h, replaced the media, and collected samples 72 h post-treatment for propidium iodide and Annexin V flow cytometry with eBioscience Annexin V apoptosis Detection kit FITC as recommended by the manufacturer. The figure shows one control and one Chernobyl cell line. The percentage of healthy cells are shown in the lower-left corner, necrotic cells in the upper-left corner, and apoptotic cells at right. (PDF 66 kb)

https://dx.doi.org/10.6084/m9.figshare.7026611