6533b86ffe1ef96bd12cd457
RESEARCH PRODUCT
Fast identification of wine related lactic acid bacteria by multiplex PCR
Helmut KönigAnna PetriJürgen FröhlichJ. Pfannebeckersubject
WineBacteriabiologyLactobacillus brevisPediococcus acidilacticiWinebiology.organism_classificationMicrobiologySpecies SpecificityLeuconostoc mesenteroidesFermentationMalolactic fermentationFermentationLactic AcidFood scienceAcetic acid bacteriaMultiplex Polymerase Chain ReactionDNA PrimersFood ScienceOenococcus oenidescription
The microflora of must and wine consists of yeasts, acetic acid bacteria and lactic acid bacteria (LAB). The latter group plays an important role for wine quality. The malolactic fermentation carried out by LAB leads to deacidification and stabilisation of wines. Nevertheless, LAB are often associated with wine spoilage. They are mainly responsible for the formation of biogenic amines. Furthermore, some strains produce exopolysaccharide slimes, acetic acid, diacetyl and other off-flavours. In this context a better monitoring of the vinification process is crucial to improve wine quality. Moreover, a lot of biodiversity studies would also profit from a fast and reliable identification method. In this study, we propose a species-specific multiplex PCR system for a rapid and simultaneous detection of 13 LAB species, frequently occurring in must or wine: Lactobacillus brevis, Lb. buchneri, Lb. curvatus, Lb. hilgardii, Lb. plantarum, Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus acidilactici, P. damnosus, P. inopinatus, P. parvulus, P. pentosaceus and Weissella paramesenteroides.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-02-01 | Food Microbiology |