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RESEARCH PRODUCT
C4 Alpha-Chain Reference Typing Report
Beate Stradmann-bellinghausenCh. Rittnersubject
AntigenicitybiologyMacromolecular Substancesmedicine.drug_classBlotting WesternImmunologyC4AAntibodies MonoclonalComplement C4chemical and pharmacologic phenomenaHematologyMonoclonal antibodyMolecular biologyGenesReference ValuesMonoclonalbiology.proteinmedicineHumansElectrophoresis Polyacrylamide GelTypingAntibodyAlpha chaindescription
Previously it was shown that C4A and C4B alpha-chains after separation on SDS-PAGE can provide valuable information on presence and absence, as well as the number of C4A and C4B genes expressed in an individual. All samples submitted for C4 reference typing were also subjected to C4 alpha-chain separation; the results were included in the Final C4 Reference Typing List [Complement Inflamm 1990;7:193-212]. In addition, in selected cases with assumed 'reversed antigenicity', Western blots of C4 alpha-chains with monoclonal anti-C4A and B antibodies were obtained. As a result, subtypic differences of C4B allotypes were detected by the comparison of monoclonal antibodies 1217 and 1228.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-01-01 | Complement and Inflammation |