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RESEARCH PRODUCT

Combination of Resveratrol with omega-3 fatty acids synergize to counteract VEGF-R pathway in sick retinal pigment epithelium cells mimicking AMD

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description

Purpose The aim of this work is to assess whether RSV (Resveratrol) can act synergistically with omega-3 fatty acids to modulate VEGF (Vascular Endothelial Growth Factor) signaling pathway in order to identify a new and more effective therapy for the treatment of AMD (Age-Related Macular Degeneration). Methods In this study, undifferentiated and differentiated human retinal pigment epithelial cells (ARPE-19) were used. The cells were treated with an omega-3/RSV preparation (Resvega®), or a RSV-free formulation or RSV alone for 24 h. The expression of key proteins in VEGF signaling pathway was evaluated by Western Blotting. Results We observed that the combination omega-3/RSV preparation (Resvega®) induces a stronger inhibition of the VEGF-R pathway activation than the RSV-free formulation or RSV alone in sick retinal cells. Surprisingly, Resvega® maintains and increases the functional VEGF-R pathway in normal retinal cells. Conclusions This work brings a new insight into the mechanism by which omega-3/RSV could counteract AMD and could protect the other eye when AMD is already present. Financial Support: This work is supported by Thea laboratory.