6533b86ffe1ef96bd12cdd17
RESEARCH PRODUCT
Identification of a 49-kDa hydrophobic cell wall mannoprotein present in velum yeast which may be implicated in velum formation
Sã©bastien BlanchetClaudine CharpentierH. Alexandresubject
Membrane GlycoproteinsSurface PropertiesBlotting WesternCellWineSaccharomyces cerevisiaeBiologyMicrobiologyYeastStainingFungal Proteinscarbohydrates (lipids)BlotCell wallElectrophoresismedicine.anatomical_structureBiochemistryCell WallBiotinylationGeneticsmedicineBiotinylationElectrophoresis Polyacrylamide GelMolecular BiologyPolyacrylamide gel electrophoresisdescription
Analysis of velum-forming yeast cell wall components released by beta-1,3-glucanase treatment were compared with those of a non velum-forming yeast. SDS-PAGE electrophoresis and Western blotting with ConA-peroxidase staining of mannoproteins allowed us to identify a 49-kDa mannoprotein present in the cell wall of the velum-forming yeast and hardly visible in the control. The cell wall nature of this protein was confirmed by labelling with the non-permeable sulfosuccinimydiyl-6-(biotinamido)hexanoate reagent. A partial purification of this mannoprotein by anion exchange HPLC followed by surface hydrophobicity determination revealed that the fraction containing the 49-kDa mannoprotein was the most hydrophobic. Since cell surface hydrophobicity plays an important role in aggregate formation, it is likely that this mannoprotein is involved in velum formation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-04-08 | FEMS Microbiology Letters |