6533b86ffe1ef96bd12ce468

RESEARCH PRODUCT

Estudio de los mecanismos de resistencia a glifosato en dos biotipos de lolium spp.

subject

description

The aim of this study was to analyse the glyphosate resistance mechanisms in Lolium multiflorum and Lolium rigidum. Both these species were collected in Spain, the former in Andalusia in olive grove crops and the latter in citrus plantations in Valencia. These were compared to a susceptible population of both species, which had never been treated with herbicides. Absorption-translocation, contact angle and retention assays were performed. The experiment design used was completely random with six replications. The study was performed as a consequence of data previously obtained for screening and dose-response trials, and which served to confirm the resistance of the resistant biotypes. Regarding the dose-response tests, these were carried out at different growth stages of the plants, with the ED50 values exhibiting an increase as their degree of growth increased. The results obtained in these trials showed that there were no significant differences in L. multiflorum. In the case of L. rigidum, there were significant differences in the foliar penetration tests, with around 20% of the 14C-glyphosate deposited on the resistant biotype being absorbed; differences in the translocation towards the root and in the retention of the glyphosate were also found.