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RESEARCH PRODUCT
Determination of triamterene in urine by HPLC using fluorescence detection and column-switching
Rosa Herráez-hernándezAdela Sevillano-cabezaP. Campíns Falcósubject
TriamtereneDetection limitAnalyteChromatographyChemistryOrganic ChemistryClinical BiochemistryUrineBiochemistryHigh-performance liquid chromatographyFluorescence spectroscopyAnalytical Chemistrychemistry.chemical_compoundmedicineAcetonitrileQuantitative analysis (chemistry)medicine.drugdescription
A liquid chromatographic method incorporating column-switching and fluorimetric detection for the determination of triamterene in untreated urine, is described. The urine samples (5 μL) were directly introduced onto an Hypersil ODS-C18, 30 μm (20 mm×2.1 mm I.D.) pre-column. Polar urinary compounds were removed by flushing the pre-column with water for 1 min, and the analyte was then switched onto an HP-LiChrospher RP C18,5 μm (125 mm×4mm ID) analytical column using an acetonitrile/phosphate buffer gradient elution. Fluorescence detection was performed at 230 nm excitation and 430 nm emission wavelengths. The recovery of drug was 102±2% in the 0.10–20.0 μg/mL concentration range, the limit of detection being 5 ng/mL. A validation of the usefulness of this procedure was accomplished by analysing urine extracts obtained from real samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-01-01 | Chromatographia |