6533b86ffe1ef96bd12ce8d1

RESEARCH PRODUCT

Immune evasion proteins gpUS2 and gpUS11 of human cytomegalovirus incompletely protect infected cells from CD8 T cell recognition

M. WillsK. BesoldBodo Plachter

subject

Human cytomegalovirusvirusesAntigen presentationIE1CytomegalovirusCD8-Positive T-LymphocytesVirus ReplicationMajor histocompatibility complexpp65US2Immediate-Early ProteinsViral Matrix ProteinsHLA-B7 AntigenInterferon-gammaViral ProteinsImmune systemViral Envelope ProteinsVirologyHLA-A2 AntigenMHC class ImedicineHumansCytotoxic T cellCells CulturedAntigen PresentationbiologyImmune evasionRNA-Binding Proteinsvirus diseasesbiochemical phenomena metabolism and nutritionPhosphoproteinsmedicine.diseaseVirologyCTL*MutagenesisCTLCytomegalovirus InfectionsMHC class Ibiology.proteinUS11CD8

description

AbstractHuman cytomegalovirus (HCMV) encodes four glycoproteins, termed gpUS2, gpUS3, gpUS6 and gpUS11 that interfere with MHC class I biosynthesis and antigen presentation. Despite gpUS2–11 expression, however, HCMV infection is efficiently controlled by cytolytic CD8 T lymphocytes (CTL). To address the role of gpUS2 and gpUS11 in antigen presentation during viral infection, HCMV mutants were generated that expressed either gpUS2 or gpUS11 alone without coexpression of the three other proteins. Fibroblasts infected with these viruses showed reduced HLA-A2 and HLA-B7 surface expression. Surprisingly, however, CTL directed against the tegument protein pp65 and the regulatory IE1 protein still recognized and lysed mutant virus infected fibroblasts. Yet, suppression of IE1 derived peptide presentation by gpUS2 or gpUS11 was far more pronounced. The results show that gpUS2 and gpUS11 alone only incompletely protect HCMV infected fibroblasts from CTL recognition and underline the importance of studying infected cells to elucidate HCMV immune evasion.

yearjournalcountryeditionlanguage
2009-08-01Virology
https://doi.org/10.1016/j.virol.2009.06.004