6533b870fe1ef96bd12cef98

RESEARCH PRODUCT

DETERMINATION OF STEROIDAL HORMONES IN URINE SAMPLES BY MICELLAR LIQUID CHROMATOGRAPHY FOLLOWING SOLID-PHASE EXTRACTION

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Steroidal hormones were determined in spiked urine samples using micellar mobile phases of sodium dodecyl sulphate (SDS)-pentanol, solid-phase extraction (SPE), and detection in the UV region. In the optimized procedure, a 10 mL aliquot of urine sample is loaded into a C18 cartridge and washed with 2 mL of 50:50 (v/v) methanol-water, followed by 200 μL of 70:30 (v/v) methanol-water. The retained steroids are eluted with 2 mL of methanol and the eluate evaporated to dryness under nitrogen at 50°C. The residue is redissolved with 200 μL of the micellar mobile phase used in the chromatographic separation and injected into the chromatograph. The performance of the procedure was checked for 13 steroidal hormones: dehydrotestosterone, methandienone, methenolone enanthate, methyltestosterone, dydrogesterone, medroxyprogesterone, medroxyprogesterone acetate, nandrolone, nandrolone decanoate, progesterone, testosterone, testosterone enanthate, and testosterone propionate. Limits of detection were below 5 ng/mL for...