6533b870fe1ef96bd12cf12d

RESEARCH PRODUCT

SELF-ASSEMBLED AMPHIPHILIC HYALURONIC ACID GRAFT COPOLYMERS FOR TARGETED RELEASE OF ANTITUMORAL DRUG

Giovanna PitarresiAntonella AlbaneseCalogero FioricaPasquale PiconeFabio Salvatore PalumboGaetano Giammona

subject

Magnetic Resonance SpectroscopySELF ASSEMBLING HYALURONIC ACID DRUG RELEASEPolymersMolecular Sequence DataPharmaceutical ScienceAntineoplastic Agentsmacromolecular substancesMicelleCell Linechemistry.chemical_compoundMiceDrug Delivery SystemsPolylactic acidCell Line TumorHyaluronic acidPEG ratioAmphiphileCopolymerOrganic chemistryAnimalsHumansHyaluronic AcidMicellesDrug CarriersChemistrytechnology industry and agricultureMicroscopy ElectronCarbohydrate SequenceMicroscopy FluorescenceSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoBiophysicsPyreneDrug carrier

description

Polymeric micelles obtained by self-assembling of amphiphilic hyaluronic acid (HA) graft copolymers have been prepared and characterized. In particular, hyaluronic acid (HA) has been grafted to polylactic acid (PLA) and polyethylenglycol chains (PEG), then the copolymers able to form micelles in aqueous medium have been chosen to entrap the antitumoral drug Doxorubicin. The critical aggregation concentration of HA-g-PLA or HA-g-PLA-g-PEG micelles has been determined by using pyrene as a fluorescent probe, whereas their shape and size have been evaluated by light scattering measurements, scanning and transmission electron microscopies. The selective cytotoxicity of drug loaded micelles toward the CD-44 over-expressing HCT-116 cells compared to receptor deficient human derm fibroblasts has been demonstrated. Pegylated micelles showed better stability and drug loading capacity and they were able to escape from macrophage phagocytosis.

yearjournalcountryeditionlanguage
2009-11-27
http://hdl.handle.net/10447/53668