6533b870fe1ef96bd12cf172
RESEARCH PRODUCT
Hyaluronic Acid Derivative with Improved Versatility for Processing and Biological Functionalization
Giovanna PitarresiAnna TamburelloGaetano GiammonaRoberto PuleioStefano AgnelloCalogero FioricaFabio Salvatore PalumboRuggero Loriasubject
Materials Chemistry2506 Metals and AlloysChemical procedurePolymers and PlasticsBioengineering02 engineering and technologyMaleimide chemistry010402 general chemistry01 natural sciencesDexamethasoneBiomaterialschemistry.chemical_compoundChondrocytesHyaluronic acidCell AdhesionmedicineMaterials ChemistryAnimalsOrganic chemistryHyaluronic AcidCell adhesionCell Proliferationchemistry.chemical_classificationRGDPolymers and PlasticTissue ScaffoldsRGD peptideDrug release021001 nanoscience & nanotechnologyHyaluronic acid derivativeBiomaterial0104 chemical scienceschemistryCollagen type IIThiolBiophysicsSurface modificationCattleLeaching (metallurgy)Swellingmedicine.symptom0210 nano-technologyPorosityBiotechnologydescription
A hydrophobic/amino functionalized derivative of hyaluronic acid (HA-EDA-C18 ) has been processed by salt leaching technique as porous scaffold without need of chemical crosslinking. Aim of this work is to demonstrate the improved versatility of HA-EDA-C18 in terms of processing and biological functionalization. In particular, the chemical procedure to tether thiol bearing RGD peptide has been described. Moreover, the possibility to load and to control the release of slightly water soluble effectors has been demonstrated by using dexamethasone. First, the swelling and degradation profiles of the scaffolds have been investigated, then the evaluation of metabolic activity of bovine chondrocytes, the histological analysis, and microscope observations has been performed to evaluate cellular adhesion and proliferation as well as the production of collagen type II.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-11-22 | Macromolecular Bioscience |