Search results for "RGD"
showing 10 items of 81 documents
RGD motifs on the surface of baculovirus enhance transduction of human lung carcinoma cells.
2006
Baculovirus vectors have been shown to enter a variety of mammalian cell lines and gene transfer with wild-type baculovirus (WT) has been demonstrated both in vitro and in vivo. Different protein motifs have been displayed on the viral surface to serve as ligands for cell-specific receptor molecules. We have generated recombinant baculovirus vectors displaying an RGD-motif, recognized by alphaV integrin, on the viral surface. The RGD motifs within the C-terminus of coxsackie virus A9 and human parechovirus 1 VP1 proteins were fused to the N-terminus of the major envelope glycoprotein, gp64, of Autographa californica multiple nucleopolyhedrovirus. The recombinant RGD-presenting viruses bound…
Effective killing of Borrelia burgdorferi in vitro with novel herbal compounds
2018
Introduction: The tick-borne disease Lyme Borreliosis is caused by Borrelia bacteria. The disease can persist even after treatment with antibiotics, which is why other methods of treatment are needed. Herbal compounds and phytochemicals have been recently examined in relation to eradicating Borrelia bacteria in vitro. Objective: The possible antimicrobial effect of two novel compounds, Biocidin Liquid and LSF Broad-Spectrum Liposomal formulas, was examined in the hopes of discovering an alternative method for eradication of Borrelia bacteria. Methods: The minimum inhibitory concentrations (MICs) and minimum bacterial deaths (MBDs), as well as, time-kill effect of each compound were utilize…
Borrelia burgdorferi ārējās virsmas proteīnu BB0689 un BBA73 funkcionālie pētījumi
2015
Maģistra darbs veidots ar mērķi noskaidrot B. burgdorferi ārējās virsmas proteīnu BB0689 un BBA73 potenciālo lomu patogēna un saimniekorganisma mijiedarbībā, kas varētu paplašināt mūsu zināšanas par Laimboreliozes patoģenēzi. Borēlijas proteīnu un zīdītāju vai artropodu proteīnu iespējamā mijiedarbība ir noteikta, izmantojot Ni-NTA afinitātes hromatogrāfiju, proteīnu kompleksu imūnprecipitāciju (Co-Ip) un attālināto Western blotu. Konstatēto mijiedarbības partneru identifikācijai ir izmantota MALDI-TOF masas spektrometrija. Pētījuma rezultātā ir konstatēta BB0689 iespējamā saistība ar vairākiem zīdītāju proteīniem, savukārt BBA73 gadījumā ir novērota tā mijiedarbība ar Ixodes ērču proteīnie…
BBE31 from the Lyme disease agent Borrelia burgdorferi, known to play an important role in successful colonization of the mammalian host, shows the a…
2019
Abstract Lyme disease is a tick-borne infection caused by Borrelia burgdorferi sensu lato complex spirochetes. The spirochete is located in the gut of the tick; as the infected tick starts the blood meal, the spirochete must travel through the hemolymph to the salivary glands, where it can spread to and infect the new host organism. In this study, we determined the crystal structures of the key outer surface protein BBE31 from B. burgdorferi and its orthologous protein BSE31 (BSPA14S_RS05060 gene product) from B. spielmanii. BBE31 is known to be important for the transfer of B. burgdorferi from the gut to the hemolymph in the tick after a tick bite. While BBE31 exerts its function by intera…
Laimas slimības ierosinātāja Borrelia burgdorferi ārējās virsmas proteīna BB0663 iegūšana un struktūras analīze
2019
Darba mērķis bija iegūt Laimas slimības izraisītāja Borrelia burgdorferi (B. burgdorgeri) celma B31 ārējās virsmas proteīna BB0663 struktūru un veikt šīs struktūras analīzi, lai iegūtu plašākas zināšanas par baktērijas patoģenēzes mehānismiem. Darba rezultātā BB0663 proteīns tika uzproducēts Escherichia coli šūnās, attīrīts un kristalizēts. Ar rengenstruktūranalīzes palīdzību tika iegūti šī kristāla difrakcijas dati. Izmantojot iegūtos datus, tika noteikta BB0663 proteīna 3D struktūra. Struktūras analīze parādīja, ka B. burgdorferi BB0663 proteīns ir līdzīgs baktērijas Novosphingobium aromaticivorans (N. aromaticivorans) proteīnam, kuram ir noteikta struktūra, bet nav zināma funkcija. Pētīj…
The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes
2017
Fibronectin (FN), a major extracellular matrix component, enables integrin-mediated cell adhesion via binding of α5β1, αIIbβ3 and αv-class integrins to an RGD-motif. An additional linkage for α5 and αIIb is the synergy site located in close proximity to the RGD motif. We report that mice with a dysfunctional FN-synergy motif (Fn1syn/syn) suffer from surprisingly mild platelet adhesion and bleeding defects due to delayed thrombus formation after vessel injury. Additional loss of β3 integrins dramatically aggravates the bleedings and severely compromises smooth muscle cell coverage of the vasculature leading to embryonic lethality. Cell-based studies revealed that the synergy site is dispensa…
Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in Ixodes ricinus ticks in Brønnøysund in northern Norway
2013
Ticks are important vectors of disease for both humans and animals. In Europe, Lyme borreliosis is the most abundant tick-borne human disease, whereas anaplasmosis, or tick-borne fever, is the most widespread tick-borne infection in domestic animals. However, knowledge about the prevalence of the causative disease agents in questing ticks in the northern range of their distribution in Norway is missing. Ixodes ricinus ticks were therefore collected by flagging vegetation in Brønnøysund, an area near the Arctic Circle in Norway where ticks have been abundant for decades. Ticks were analysed for infection with Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum by real-time PCR ampl…
Host dispersal shapes the population structure of a tick-borne bacterial pathogen
2020
Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick-borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale. We collected ticks from passerine birds in 11 European countries. B. burgdorferi s.l. prevalence in Ixodes spp. was 37% and increased with latitude. The fieldfare Turdus pilaris and the blackbird T. merula carried ticks with the highest Borrelia prevale…
Sympatric Ixodes-tick species: pattern of distribution and pathogen transmission within wild rodent populations
2018
AbstractThe generalist tick Ixodes ricinus is the most important vector for tick-borne pathogens (TBP), including Borrelia burgdorferi sensu lato, in Europe. However, the involvement of other sympatric Ixodes ticks, such as the specialist vole tick I. trianguliceps, in the enzootic circulations of TBP remains unclear. We studied the distribution of I. ricinus and I. trianguliceps in Central Finland and estimated the TBP infection likelihood in the most common rodent host in relation with the abundance of the two tick species. Ixodes trianguliceps was encountered in all 16 study sites whereas I. ricinus was frequently observed only at a quarter of the study sites. The abundance of I. ricinus…
Characterization of dry-film photolithography for the study of B. burgdorferi motility
2017
Kuivafilmilitografian karakterisointi Borrelia burgdorferi:n liikkuvuuden tutkimiselle. Borrelia burgdorferi on puutiaisten kantama spirokeettabakteeri, joka voi tarttua useisiin eläinlajeihin. Ihmisillä B. burgdorferi aiheuttaa borrelioosia. Tauti on laajalle levinnyt monissa pohjois- ja länsimaissa ja leviää entisestään ilmastonmuutoksen laajentaessa punkkien elinaluetta. Monet tekijät vaikeuttavat borrelioosin hoitoa. B. burgdorferin kyky välttää ihmisen luontaista immuunijärjestelmää ovat tässä suhteessa merkittävä. Bakteerien liikkuvuus on olennainen tämän kyvyn kannalta, mutta sen tarkka rooli bakteerien kyvyssä liikkua verisuonistossa on osittain tuntematon. Mikrofluidistiikka hyödyn…