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RESEARCH PRODUCT
Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction
Rafael PayáPilar SepúlvedaMauro LlopVicente MirabetAmparo RuizAna ArmiñánElisa LledóFrancisco Carbonell-uberosJosé Manuel García-verdugoJorge Sanchez-torrijosM Dolores MiñanaJosé Anastasio MonteroCarolina Gandíasubject
AdultMalePathologymedicine.medical_specialtyAdolescentAngiogenesismedicine.medical_treatmentMyocytes Smooth MuscleCell- and Tissue-Based TherapyMyocardial InfarctionNeovascularization PhysiologicBiologystem cell therapyventricular remodelingVentricular Function LeftRats Nudeleft ventricular functionDental pulp stem cellsmedicineAnimalsHumansMyocytes CardiacMyocardial infarctionVentricular remodelingDental PulpCell ProliferationUltrasonographymesenchymal stem cellsStem CellsCardiac muscleCell DifferentiationMesenchymal Stem CellsAmniotic stem cellsCell BiologyStem-cell therapyAnatomymedicine.diseasedental pulp stem cellsRatsRetroviridaemedicine.anatomical_structureMolecular MedicineStem cellRetroviridae InfectionsStem Cell TransplantationDevelopmental Biologydescription
Abstract Human dental pulp contains precursor cells termed dental pulp stem cells (DPSC) that show self-renewal and multilineage differentiation and also secrete multiple proangiogenic and antiapoptotic factors. To examine whether these cells could have therapeutic potential in the repair of myocardial infarction (MI), DPSC were infected with a retrovirus encoding the green fluorescent protein (GFP) and expanded ex vivo. Seven days after induction of myocardial infarction by coronary artery ligation, 1.5 × 106 GFP-DPSC were injected intramyocardially in nude rats. At 4 weeks, cell-treated animals showed an improvement in cardiac function, observed by percentage changes in anterior wall thickening left ventricular fractional area change, in parallel with a reduction in infarct size. No histologic evidence was seen of GFP+ endothelial cells, smooth muscle cells, or cardiac muscle cells within the infarct. However, angiogenesis was increased relative to control-treated animals. Taken together, these data suggest that DPSC could provide a novel alternative cell population for cardiac repair, at least in the setting of acute MI. Disclosure of potential conflicts of interest is found at the end of this article.
year | journal | country | edition | language |
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2008-01-01 | Stem Cells |