6533b870fe1ef96bd12cf2df

RESEARCH PRODUCT

Determination of sulphonamides in human urine by azo dye precolumn derivatization and micellar liquid chromatography

Guillermo Ramis-ramosErnesto F. Simó-alfonsoJosep Esteve-romeroM.c. García-alvarez-coque

subject

Detection limitSulfonamidesChromatographySodiumchemistry.chemical_elementGeneral ChemistryUrineHigh-performance liquid chromatographychemistry.chemical_compoundSpectrometry FluorescenceAnti-Infective AgentschemistryReference ValuesMicellar liquid chromatographyHumansIndicators and ReagentsSpectrophotometry UltravioletNitriteDerivatizationAzo CompoundsChromatography High Pressure LiquidMicellesAntibacterial agent

description

Abstract A high-performance liquid chromatographic method for the determination of sulphonamides in urine is reported. The drugs (sulphadiazine, sulphaguanidine, sulphamethizole, sulphamethoxazole, and sulphathiazole) were diazotized with nitrite and coupled with N-(1-naphthyl)ethylenediamine dihydrochloride in a sodium dodecyl sulphate (SDS) micellar medium. Separation of the sulphonamide azo dyes was performed on a C18 column with a 0.05 M SDS-2.4% pentanol mobile phase, which permitted the direct injection of the urine samples. The limits of detection were in the 0.1–0.3 μg/ml range.

yearjournalcountryeditionlanguage
1995-08-04Journal of Chromatography B: Biomedical Sciences and Applications
https://doi.org/10.1016/0378-4347(95)00158-f