MUSCLE BIOENERGETICS IN OBESE ZUCKER RATS

6533b870fe1ef96bd12cf2e9

RESEARCH PRODUCT

MUSCLE BIOENERGETICS IN OBESE ZUCKER RATS

Marc Klein Paul Walker J. Straczek F Barbé Pierre Kaminsky M. Duc Claude Burlet

subject

medicine.medical_specialty Magnetic Resonance Spectroscopy Physiology Endocrinology Diabetes and Metabolism Intracellular pH PHOSPHOCREATINE [INFO.INFO-IM] Computer Science [cs]/Medical Imaging Stimulation 030204 cardiovascular system & hematology Biology Phosphates Phosphocreatine 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine MITOCHONDRIA GENETIC OBESITY Physiology (medical)Internal medicine MUSCLES medicine [INFO.INFO-IM]Computer Science [cs]/Medical Imaging Animals NUCLEAR MAGNETIC RESONANCE Obesity Acidosis 2. Zero hunger Analysis of Variance [ INFO.INFO-IM ] Computer Science [cs]/Medical Imaging ADENOSINE 5'-TRIPHOSPHATE ADENOSINE 5'-DIPHOSPHATE Metabolism Electric Stimulation Rats Rats Zucker Adenosine Diphosphate Adenosine diphosphate Endocrinology chemistry ENERGETIC METABOLISM INTRACELLULAR PH Female medicine.symptom Energy Metabolism Adenosine triphosphate 030217 neurology & neurosurgery Muscle Contraction Muscle contraction

description

International audience; Abstract: The purpose of this study was to investigate the energetic metabolism in obese Zucker rats, using phosphorus nuclear magnetic resonance spectroscopy at rest and during a 2-Hz muscle stimulation and subsequent recovery. Animals were anesthetized with ketamine (150 mg/kg ip). Fed obese rats and 2-day-fasted obese rats were compared with their normally fed and 2-day-fasted lean litter mates. No differences were found between the two groups for ATP, total creatine, phosphocreatine (PCr), and intracellular pH. Starvation in lean rats resulted in a significant fall in inorganic phosphate (Pi), increased resting ADP level, and decreased PCr and ADP recovery after stimulation. The obese rats exhibited a decreased PCr/Pi and increased ADP at rest and a decreased PCr resynthesis and ADP metabolization rate after stimulation. Muscle stimulation in fasted obese rats induced higher PCr depletion and more pronounced acidosis. These results suggest an in vivo mitochondrial metabolism dysfunction in fasted lean as well as in fed and fasted obese rats.

year	journal	country	edition	language
1994-03-01

https://hal.archives-ouvertes.fr/hal-00790168