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RESEARCH PRODUCT

Genomic and functional gene studies suggest a key role of beta-carotene oxygenase 1 like (bco1l) gene in salmon flesh color

Thomas MoenHanna HelgelandHanna HelgelandNina ZoricSigbjørn LienJohannes Von LintigFabian GrammesDag Inge VågeSissel KjøglumMarte SodelandMarte SodelandJacob Seilø Torgersen

subject

0301 basic medicineOxygenasegenetic structuresQuantitative Trait LociSalmo salarPopulationlcsh:MedicineGenome-wide association studySingle-nucleotide polymorphismQuantitative trait locusBiologyArticle03 medical and health sciencesstomatognathic systemGenetic variationAnimalsVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923lcsh:ScienceeducationCarotenoidGenebeta-Carotene 1515'-Monooxygenasechemistry.chemical_classificationGeneticseducation.field_of_studyMultidisciplinary030102 biochemistry & molecular biologyPigmentationEukaryoteFleshlcsh:Rfood and beveragesGenomicsbeta CaroteneEnzymes030104 developmental biologychemistrylcsh:QGenome-Wide Association Study

description

AbstractRed coloration of muscle tissue (flesh) is a unique trait in several salmonid genera, including Atlantic salmon. The color results from dietary carotenoids deposited in the flesh, whereas the color intensity is affected both by diet and genetic components. Herein we report on a genome-wide association study (GWAS) to identify genetic variation underlying this trait. Two SNPs on ssa26 showed strong associations to the flesh color in salmon. Two genes known to be involved in carotenoid metabolism were located in this QTL- region: beta-carotene oxygenase 1 (bco1) and beta-carotene oxygenase 1 like (bco1l). To determine whether flesh color variation is caused by one, or both, of these genes, functional studies were carried out including mRNA and protein expression in fish with red and pale flesh color. The catalytic abilities of these two genes were also tested with different carotenoids. Our results suggest bco1l to be the most likely gene to explain the flesh color variation observed in this population.

yearjournalcountryeditionlanguage
2019-10-29
10.1038/s41598-019-56438-3https://hdl.handle.net/11250/2680141