6533b870fe1ef96bd12cf95d
RESEARCH PRODUCT
Processing and Presentation of Protein and Parasite-Derived Antigens by 4F7+ Dendritic Cells
Jürgen KnopGemot GradehandtErwin RüdeMansour MohamadzadehAnastassia Pavlidousubject
MHC class IIbiologyAntigenFollicular dendritic cellsChemistryAntigen processingAntigen presentationbiology.proteinDendritic cellAntigen-presenting cellMolecular biologyEpitopedescription
The dendritic cell (DC), a trace component of splenocytes is the principal cell type required for a primary mixed lymphocyte reaction.1 Splenic DC are described as antigen presenting cells (APC) capable to generate immunogenic fragments of intact protein antigens for presentation to MHC class II restricted T cells,2 in contrast to former findings.3 Langerhans cells (LC) as potent APC are members of the dendritic cell lineage forming a system of potent APC, first identified by Steinman and Conn.4 Importanly Schuler and Steinman have subsequently presented experimental evidence suggesting that LC represent immature DC.3 During infectious diseases caused by parasites of the genus Leishmania LC of skin capture, process, and transport antigen to the regional lymph nodes for subsequent presentation to specific T cells.5 LC also play a critical role as APC in promoting contact hypersensitivity reactions. In order to obtain monoclonal antibodies (mAb) for characterization of DC involved in the induction and elicication of allergic contact dermatitis DC-specific mAb were selected. Rats were immunized with Ia+ epidermal cells from BALB/c mice epicutanously treated with the contact sensitizer 2,4-dinitrofluorbenzene. The mAb 4F7 was generated and was found to recognize an epitope expressed on DC from various tissues.6,7,8
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-01-01 |