6533b870fe1ef96bd12cfa96

RESEARCH PRODUCT

Organometallic complexes with biological molecules: V.In vivo cytotoxicity of diorganotin(IV)-amoxicillin derivatives in mitotic chromosomes ofrutilus rubilio (pisces, cyprinidae)

subject

description

In order to test in vivo cytotoxicity of diorganotin(IV)-amoxicillin (amox) derivatives, mitotic chromosomes of Rutilus rubilio (Pisces, Cyprinidae) have been analyzed using two different chromosome-staining techniques. Results gathered after exposure of fish to the free amox.3H 2 O, R 2 SnClamox.2H 2 O, and R 2 Snamox 2 .2H 2 O (R = methyl, butyl and phenyl ; amox - = 6-[D(-)-β-amino-p-hydroxyphenylacetamido]penicillinate) suggest that methyl derivatives seem to exert a lower cytotoxicity than butyl and phenyl ones and that R 2 Snamox 2 .2H 2 O derivatives are more toxic than R 2 Snclamox.2H 2 O at both 10 -5 and 10 -7 mol dm -3 concentrations. The following structural lesions have been identified by comparative analysis of mitotic chromosomes from untreated specimens (controls) and specimens treated with diorganotin(IV)-amoxicillin derivatives : (1) differentially stained chromosome areas ; (2) granular deeply stained zones along the chromosomal body ; (3) arm breakages; and (4) side-arm bridges (pseudochiasmata).