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RESEARCH PRODUCT
Induction of B-cell development in adult mice reveals the ability of bone marrow to produce B-1a cells
Elias HobeikaThorsten BuchKarsten KretschmerSiegfried WeissBishnudeo RoyMartina KreyAri WaismanMartin HafnerStefan LienenklausMichael RethSandra Dübersubject
Adoptive cell transfer1303 BiochemistryGenes RAG-1Immunology2720 HematologyB-Lymphocyte SubsetsSpleenBone Marrow CellsEnzyme-Linked Immunosorbent AssayMice Transgenic610 Medicine & healthBiology10263 Institute of Experimental ImmunologyBiochemistryPolymerase Chain ReactionRecombination-activating gene1307 Cell BiologyPeritoneal cavityMicemedicineAnimalsB cellB-Lymphocytes2403 ImmunologyStem CellsCell DifferentiationCell BiologyHematologyMarginal zoneFlow CytometryMolecular biologyAdoptive Transfermedicine.anatomical_structureImmunoglobulin MImmunologybiology.protein570 Life sciences; biologyBone marrowdescription
AbstractTo study B-cell development from bone marrow (BM), we generated recombination-activating gene 1 (Rag1)–targeted mice lacking mature lymphocytes. B-cell development can be induced in such mice by B cell–specific restoration of a functional Rag1 transcription unit. Follicular and marginal zone B cells populated the spleen when Rag1 expression was permitted. Notably, the peritoneal cavity was dominated by bona fide B-1a cells, as judged by surface markers and functional properties. These BM-derived B-1a cells exhibited a polyclonal VDJ repertoire with substantial N nucleotide insertions. Nevertheless, physiologic frequencies of phosphatidylcholine-specific B cells were detected. Importantly, the BM of young and 5-month-old mice was indistinguishable with regard to the potential to generate B-1a cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-12-03 |