6533b870fe1ef96bd12cfcc2
RESEARCH PRODUCT
Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy
Jorge Garcia-sucerquiaEmilio Sánchez-ortigaManuel Martínez-corralAna DoblasGenaro Saavedrasubject
ErythrocytesMaterials scienceMicroscopeHolographyBiomedical EngineeringHolographyMagnificationImage processinglaw.inventionBiomaterialsBiological specimenOpticslawMicroscopyImage Processing Computer-AssistedAnimalsMicroscopybusiness.industryÒpticaAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsÒptica Aparells i instrumentsDrosophila melanogasterDigital holographic microscopybusinessHeadDigital holographydescription
The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 |